Principal Scientist – GEM Molecular Validation & Technologies

Job description

Career CategoryScientificJob DescriptionJoin Amgen’s Mission of Serving PatientsAt Amgen, if you feel like you’re part of something bigger, it’s because you are. Our shared mission—to serve patients living with serious illnesses—drives all that we do. Since 1980, we’ve helped pioneer the world of biotech in our fight against the world’s toughest diseases. With our focus on four therapeutic areas –Oncology, Inflammation, General Medicine, and Rare Disease– we reach millions of patients each year. Amgen is advancing a broad and deep pipeline of medicines to treat cancer, heart disease, inflammatory conditions, rare diseases, and obesity and obesity-related conditions. As a member of the Amgen team, you’ll help make a lasting impact on the lives of patients as we research, manufacture, and deliver innovative medicines to help people live longer, fuller happier lives. Our award-winning culture is collaborative, innovative, and science based. If you have a passion for challenges and the opportunities that lay within them, you’ll thrive as part of the Amgen team. Join us and transform the lives of patients while transforming your career. Principal Scientist – GEM Molecular Validation & TechnologiesWhat you will doLet’s do this. Let’s change the world. We are seeking an experienced Principal Scientist to support and advance the Amgen Comparative Medicine Genetically Engineered Models (GEM) Program. This role will provide scientific and technical expertise in the molecular characterization and qualification of genetically engineered models, supporting model integrity from generation through colony establishment and experimental use. The individual will also contribute to building scalable GEM capabilities through sequencing, automation, computational approaches, and emerging AI-enabled tools. The ideal candidate will bring extensive experience with genetically engineered models, genome engineering, and molecular validation, with the scientific judgment to evaluate complex engineered alleles and troubleshoot unexpected molecular or genotype findings. Strong hands-on expertise in CRISPR-based model generation and characterization, qPCR/ddPCR, long-read sequencing, NGS, automation, bioinformatics, and computational analysis is highly desirable. Experience with engineered cell systems, including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), would be an additional strength. A major focus will be developing fit-for-purpose GEM molecular characterization strategies, including orthogonal genotyping, allele and structural integrity, copy number and zygosity, mosaicism, off-target analysis, and sequencing. The role will also advance scalable automation, computational, and AI-enabled approaches for model design, sequence analysis, data interpretation, and workflow integration to improve GEM quality, efficiency, and scientific decision-making. This is a highly hands-on Principal Scientist role requiring deep technical expertise, independent scientific judgment, innovation, and strong problem-solving skills. The individual will collaborate across internal and external teams to resolve complex model questions and ensure GEMs are appropriately characterized for their intended scientific use. Key Responsibilities: GEM Molecular Characterization & ValidationDevelop and execute fit-for-purpose molecular characterization strategies for newly generated and existing genetically engineered models, including knockout, knock-in, conditional, transgenic, humanized, and complex multi-allelic models. Design, develop, validate, and troubleshoot qPCR, multiplex ddPCR, long-read sequencing, NGS, and other orthogonal molecular assays. Characterize engineered alleles for sequence and structural integrity, copy number, zygosity, mosaicism, insertion sites, off-target effects, and unintended editing events. Resolve complex or inconclusive molecular and genotyping results by integrating complementary analytical approaches. Establish reproducible QC standards and workflows for internal GEM characterization. Advanced Sequencing & Expression CharacterizationDevelop and optimize Oxford Nanopore long-read sequencing and NGS workflows to characterize engineered alleles, structural variants, insertion sites, and complex genome-editing outcomes. Develop quantitative expression approaches, including RT-ddPCR and related assays, to assess the molecular consequences and expression of engineered alleles. Integrate genotype, sequence, and expression data to provide high-confidence characterization and interpretation of GEMs. Evaluate emerging molecular and sequencing technologies that improve scientific rigor, scalability, and turnaround time. Computational, AI & Data AnalysisDevelop and implement scalable computational workflows for sequencing analysis, QC, visualization, interpretation, and reporting. Apply programming, bioinformatics, and AI-enabled approaches to model design, sequence and off-target analysis, molecular data interpretation, troubleshooting, and workflow automation. Partner with computational and bioinformatics scientists to integrate molecular characterization data and develop standardized analytical pipelines. Identify opportunities to use AI and automation to improve scientific decision-making, efficiency, and scalability across the GEM lifecycle. Automation & Technology DevelopmentDevelop automated and high-throughput workflows for genotyping, sequencing, and molecular characterization. Optimize laboratory instrumentation and automation to improve throughput, reproducibility, data quality, and efficiency. Evaluate and implement new technologies that expand GEM characterization capabilities. GEM Scientific Infrastructure & CollaborationContribute to scalable GEM laboratory, data, and analytical infrastructure, including SOPs, QC checkpoints, documentation, and traceability standards. Work closely with the GEM Lead, Comparative Medicine, Therapeutic Area scientists, computational teams, Functional Genomics, and external partners to resolve complex model questions. Serve as a scientific and technical resource for GEM molecular characterization, genome engineering, sequencing, and genotype interpretation. Communicate complex molecular findings clearly to support model selection, troubleshooting, and scientific decision-making, and contribute to training and knowledge transfer. Scientific & Animal-Welfare StewardshipApply rigorous early molecular characterization to identify model issues before unnecessary colony expansion or downstream experimental investment. Ensure work aligns with animal-welfare considerations and the 3Rs principles of Replacement, Reduction, and Refinement. Contribute to continuous improvement of GEM technologies, workflows, and scientific standards. What we expect of youWe are all different, yet we all use our unique contributions to serve patients. The dynamic professional we seek is a leader with these qualifications. Basic Qualifications: Doctorate degree [and relevant post-doc where applicable] in Molecular Biology, Genetics, Cell Biology, Bioinformatics, or a related scientific discipline and 2 years of relevant research and industry experience OR Master’s degree in Molecular Biology, Genetics, Cell Biology, Bioinformatics, or a related scientific discipline and 5 years of relevant research and industry experience OR Bachelor’s degree in Molecular Biology, Genetics, Cell Biology, Bioinformatics, or a related scientific discipline and 7 years of relevant research and industry experience Preferred Qualifications: Extensive experience with genetically engineered models, genome engineering, and molecular characterization, including knockout, knock-in, conditional, transgenic, humanized, and/or complex engineered models. Strong hands-on expertise in qPCR, multiplex ddPCR, Oxford Nanopore long-read sequencing, NGS, and sequencing-based characterization. Experience with CRISPR/Cas9 model design and generation, engineered-allele validation,